Studies on production characterization and application potential of cholesterol oxidase from rhodococcus sp ncim 2891

Abstract

Cholesterol oxidase EC 1 1 3 6 is a flavoenzyme that in most cases catalyses the oxidation of cholesterol to 4 cholesten 3 one CEO cholestenone The product cholestenone has been used as a precursor for the synthesis of hormones and many intermediate steroidal compounds which are eventually used to produce anabolic drugs and contraceptive Classical one variable at a time and statistical methods Plackett Burman and Central composite design were used to optimize growth medium for the pro