Estimation of antimicrobial bacteria and fungi efficiency from the extract of exoskeleton of locally available prawn and crab species

Abstract

The current investigation submitted under the title The antimicrobial and preservative efficiency of chitosan extracted from Barytelphusa guerini (crab) and Macrobrachium lamarrei (prawn) shell , incorporates the results of studies including chemical extraction, functional properties, characterisationand antimicrobial and preservative activity of chitosan.Chitin is the world s second most abundant naturally occurring polysaccharide. Due to its origin as a key material in the external protective structure of living systems, chitin and chitosan have several outstanding property In the current research, after the demineralization, deproteinization, decoloration and deacetylation, it was found that the chitin content of prawn and crab was 17.62 and 94.14% of the dry weight, respectively. The maximum yield of chitosan was obtained from crab shell waste was recorded as 87.29% and 9.93% of total chitosan yield was recorded with prawn shell wastes.After extraction, percentage yield determination and solubility test, Degree of deacetylation (DD) is an important parameter of chitosan.In this study, DDA was determined by the acid-based titration method.The FTIR analysis has confirmed the successful extraction of chitosan from different starting materials such as crab and prawn shell. And it concluded that, the chitosan synthesized from crab and prawn spectrum is very similar to that of commercial chitosan. newline