Role of tocopherol copper and or flubendiamide induced caronic toxicity in Rats with special reference to male reproductive system

Abstract

Present study was undertaken to evaluate the ameliorative effect of and#945;-tocopherol (100 mg/kg) against copper (33 newlinemg/kg) and/or flubendiamide (200 mg/k)-induced toxicity following 90 days oral exposure. Fifty four male rats newlineof 130-150 g were divided into nine groups of six animals each. Rats of group I served as negative control and newlinegroup II as vehicle control (corn oil) while animals of groups III to IX received orally and#945;-tocopherol (100 mg/kg), newlinecopper sulphate (33 mg/kg), flubendiamide (200 mg/kg), flubendiamide (200 mg/kg) + copper sulphate (33 newlinemg/kg), copper sulphate (33 mg/kg) + and#945;-tocopherol (100 mg/kg), flubendiamide (200 mg/kg) + and#945;-tocopherol newline(100 mg/kg) and flubendiamide (200 mg/kg) + copper sulphate (33 mg/kg) + and#945;-tocopherol (100 mg/kg), newlinerespectively. Both the xenobiotics failed to produce any apparent clinical sign of toxicity or mortality in rats. newlineBody weight and per cent weight gain in rats of copper and flubendiamide alone groups and in combination newlinetreated (copper + flubendiamide) rats were markedly lower. But organ weights and relative organ weight did not newlinediffer between different treatment groups except significant increase in relative weight of lungs and brain in rats newlineof copper and copper + flubendiamide treatment groups. No significant alterations were observed in feed and newlinewater intake in any of the treatment groups except significant reduction in feed intake during 9th and 11th weeks newlinein copper-treated rats. Significant reduction in Hb, PCV, WBCs count and granulocytes count was observed in newlineflubendiamide and copper + flubendiamide treated rats while lymphocytes % was significantly increased in rats newlineof flubendiamide alone treated. RBCs, Hb, MCV, MCH, MCHC, LY%, MO%, GR% and PCT values did not newlinediffer significantly in any of the xenobiotics treatment groups except significant increase in RDWC and newlinedecrease and PLT count, respectively in copper or flubendiamide or copper + flubendiamide-treated rats. Total newlineproteins, albumin and globulin, glucose, cholesterol and HDL-cholesterol, AST and