Bioconversion Of Tannins To Gallic Acid Using Bacterial Tannase

Abstract

newlineTannin acyl hydrolase (E.C.3.1.1.20) universally known as tannase is an inducible newlineenzyme that predominantly acts on tannins. Tannins have been documented as high newlinemolecular weight polyphenolic compounds possessing molecular weight in the range newline(500 3000 kDa). Tannins are the second most abundant polyphenolic compounds newlineexisting in nature after lignin. Tannins are water-soluble secondary metabolites newlineexisting in abundance in plants. Tannase specifically acts upon ester and depside newlinelinkages in hydrolysable tannins particularly the gallotannins thus releasing gallic acid newlineand glucose molecule. Tannases have a vast range of applications in various industrial newlinebioprocesses ranging from food, feed to chemical as well as pharma sector. One of the newlinemajor applications of tannase is in the production of gallic acid. Gallic acid, the major newlinehydrolytic product of hydrolysable tannins (particularly gallotannins) has extended its newlineutilization as a highly valuable therapeutic agent: as an antimicrobial, antiviral, newlineantitumor and radio protective agent as well as a potential drug. In the present newlineresearch investigation, a potent tannase producing bacteria Bacillus haynesii strain newlineSSRY4 MN03124 was isolated from the rhizosphere of Casia fistula through primary newlineand secondary screening