Isolation and identification of ESBL Producing E Coli from various Clinical samples by phenotypic and Genotypic methods

Abstract

BACKGROUND: An important feature that complicates the management of Escherichia newline coli infection is the increased resistance to first-line antibiotics. Resistance to broad-spectrum newline beta-lactams, mediated by extended-spectrum beta-lactamase (ESBL) an increasing problem newline in the present world. newline AIMS and OBJECTIVES: To determine the phenotypic and genotypic characteristics of newline ESBL producer E.coli from clinical samples come to the diagnostic centre for investigation. newline METHODS: Thisdescriptive study was carried out in our laboratory estimation of ESBL by newline Phenotypic methods Screening test by Double disc synergy test (DDST) and confirmatory newline method, Phenotypic confirmatory disc diffusion test (PCDDT). Drug resistance patterns and newline genotypic method by RT PCR molecular method. newline 1 newline RESULTS: During this study period, 400 Escherichia coli were isolated from various newline clinical specimens of patients of all ages and both sexes out of the total isolates of newline Escherichia coli, 250 (62.5%) isolates ESBL producing E.coli and 150 (37.5%) Non-ESBL newline producing E.coli. Out of 400 E.coli, Phenotypic screening ESBL producer E.coli were newline Phenotypic confirmatory Double Disk diffusion method (PCDDT) 275 (68.7%) newline Producing E.coli, While Double Disk Synergy Test (DDST) 250 (62.5%). DDST is a reliable newline method for ESBL detection compared to other methods. Among total ESBL producers of newline Escherichia coli, 75.7% showed the presence of at least one ESBL gene. Out of ESBL newlinepositive E.coli, 189 (75.7%) were carrying NDM, 129 (51.6%) blaKPC, 123 (49.2%) newline and (87.1%) blaTEM genes, while fewer genes identify by genetically 99 (39.5%) blaCTX newlineMand54(21.5%) blaSHV isolates included in this study. newline CONCLUSIONS: Finally, other studies are needed from other healthcare settings in India to newline determine the immensity of the problem of antimicrobial resistance existing in emerging newline pathogenic bacteria like E. coli newline