To study developmental potential of goat tetraploid embryos derived from in vitro fertilization

Abstract

The present study was designed to evaluate the effects of different electro-fusion field newlinestrength (kV/cm) and pulse duration (µs) on development of goat tetraploid embryos. The effect of newlinedifferent O2 concentrations was also evaluated on the production of tetraploid embryos. For newlineproduction of tetraploid embryo, 2-cell embryos were obtained from two sources; first by the method newlineof activation of oocytes using calcium ionophores and second by the method of in-vitro fertilization. newlineThe results of the present study revealed that the cleavage rate from the parthenogenetic activation newlinewas recorded 61.87%. Out of total cleavage, the embryos which developed upto the stage of morula newlineand blastocyst were 11.99% and 2.49%, respectively. The cleavage rate of in-vitro fertilized oocytes newlinewas 14.8%. Out of total cleavage, the embryos which developed upto the stage of morula and newlineblastocyst were 4.29% and 0.92%, respectively. The produced 2-cell embryos were exposed to newlinedifferent field strength and pulse duration and their fusion rate and subsequent developmental stages newlinewere compared. Optimum cleavage was obtained at the 1.2 kV/cm for 4 µs duration, from activation newlinederived 2-cell embryos (58.97%) as well as from IVF derived 2-cell embryos (33.33%). When the newlineeffect of different oxygen concentrations was evaluated, it was observed that maximum cleavage newline(82.35%) was recorded at the 5% oxygen concentration and 4.76% of the embryos developed upto the newlineblastocyst stage. It was concluded that 1.2 kV/cm field strength and 4 µs pulse duration and 5% newlineoxygen concentration were the most suitable treatment for production of tetraploid embryos. newline