Comparison of receptor of advanced glycation end products rages in tissue samples of patients with oral submucous fibrosis at various grades leukoplakia at various histological grades with oral squamous cell carcinoma

Abstract

The aim of this study is to detect and compare the levels of Receptors of newlineAdvanced Glycation End Products in tissue samples of patients with newlinehistopathologically confirmed Oral Submucous Fibrosis at various stages, Oral newlineLeukoplakia at various stages with Oral Squamous Cell Carcinoma patients in newlinetissues samples using ELISA. newline12 of 130 newlineABSTRACT newlineOBJECTIVE: newline1. To detect the levels of RAGEs in tissue samples of patients with newlinehistopathologically confirmed; various grades of Oral Submucous newlineFibrosis. newline2. To detect the levels of RAGEs in tissue samples of patients with. newlinehistopathologically confirmed; various grades of Hyperkeratosis With newlineEpithelial Dysplasia (Leukoplakia). newline3. To compare the levels of RAGEs in patients with various grades of newlineOral Submucous Fibrosis, various grades of Hyperkeratosis With newlineEpithelial Dysplasia, and Oral Squamous Cell Carcinoma. newline4. To predict the carcinogenesis in Oral Premalignant Disorders newlineMATERIALS AND METHODS: newlineA cross-sectional study was conducted among 49 patients, who newlinewere divided into 3 groups with 16 patients per group of Hyperkeratosis newlinewith Epithelial dysplasia and 16 of Oral Submucous Fibrosis and 17 Well newlineDifferentiated Squamous Cell Carcinoma. Group I included tissue newlinesamples from Well Differentiated Squamous Cell Carcinoma, group II Oral newline13 of 130 newlineABSTRACT newlineSubmucous Fibrosis at various stages, and group III Hyperkeratosis with newlineEpithelial Dysplasia at various stages. The protocol of the study was newlineapproved by the Scientific Review Board and it conforms to the newlineprovisions of the declaration of Helsinki. newlineTissue samples were then obtained from the study subjects. newlineFollowing which the tissue was subjected to homogenization and newlinecentrifugation. The supernatant was collected and subjected to ELISA newlineanalysis to detect the concentration of RAGE. newlineRESULTS: newlineIn our study we have observed that the concentration of RAGEs was newlinefound to be more at various stages of OSMF (plt0.05), which was considered newlinestatistically significant.