Syntheisis Characterization Antimicrobial and Toxicological Effect of Metal Oxides Nanoparticles

Abstract

The metal oxides nanoparticles (ZnO and Fe2O3) series were hydrothermally synthesized by maintaining specific temperature and pressure. The series of these synthesized MONPs (ZnOEU1 NPs, ZnOEU2 NPs, Fe2O3EU1 NPs and Fe2O3EU2 NPs) were characterized with the help of certain characterizing techniques; XRD, RAMAN, EDX, SEM, TEM, and FTIR. The antimicrobial effects of these four different series of MONPs were analyzed on post-harvest losses causing fungi and bacteria. The two fungi and two bacteria were isolated from post-harvested capsicum and tomato. The fungi and bacteria namely; Fusarium incarnatum (MT682502) and Staphylococcus aureus (OP114236) were isolated from capsicum, and Aspergillus niger (MT675916) and Klebsiella pneumoniae (ON406436) were isolated from tomato, which were identified by using molecular analysis techniques. The best two antimicrobial activity showing MONPs were further used for continuation of post-harvest storage trial. The ZnOEU2 NPs and Fe2O3EU2 NPs along with ethylcellulose were used to dipcoat capsicum and tomato for storage trial. The capsicum and tomato samples coated with ZnOEU2 NPs and Fe2O3EU2 NPs were analyzed for physical (weight, length, width), proximate (moisture content, crude fat, crude fiber, ash content), and biochemical (ascorbic acid, chlorophyll, lycopene). The coated capsicum samples were analyzed on day 1, 7, 14, while the coated tomato samples were analyzed on day 4, 8, 12, 16. The ZnOEU2 and Fe2O3EU2 NPs, which were used for storage study trials, had been analyzed for cytotoxicity assay by using HEK293 cell lines. newline