Bioactivity guided approach to evaluate the protective effect of Randia Dumetorum Lamk against acute lung injury

Abstract

Acute Lung Injury (ALI) and its more severe form Acute Respiratory Distress Syndrome (ARDS) is a clinical syndrome with a high mortality rate (27-41%). There is no effective pharmacological therapy available against ALI till date. So, the present work was undertaken to evaluate the anti-inflammatory effect of a traditional plant Randia dumetorum Lamk. against HCl-induced ALI in mice and unveil the key bioactive constituents possessing anti-inflammatory action by employing bioactivity-guided fractionation. The study summarized that the fruits of R. dumetorum have a potential anti-inflammatory activity against HCl-induced ALI, and oleanolic acid seems to be a major compound behind exerting such action. The glycosides/saponins might also be responsible for the anti-inflammatory action with oleanolic acid as the aglycone moiety as revealed by LC-MS-MS data and phytochemical analysis. Overall, our study demonstrates that oleanolic acid might be a suitable candidate for amelioration of acute lung injury. newline