Assessment of Microflora and MIR155 in Subgingival Plaque and Placental Samples of Preeclamaptic Pregnant Women with Chronic Periodontitis A Genetic Diagnostic Approach

Abstract

Background and Objective: Periodontal diseases can serve as reservoirs of organisms and generate systemic inflammation. To explore further, we aimed to associate periodontal viruses and bacteria with mir155 levels in dental plaque and placental tissues of preeclamptic women. newlineMaterials and Methods: We selected 450 pregnant women, aged 18-35 years and divided them to four groups. Controls- 145 systemically and periodontally healthy, Group A-100Systemically healthy women with chronic periodontitis,Group B- 100preeclamptic women with chronic periodontitis, Group C-100 preeclamptic women withoutchronic periodontitis. Age, BMI, SES, PI, BOP, PPD, CAL were recorded. newlineSubgingival plaque and placental tissues (postpartum) were collected and assessed for periodontal pathogens such as Tannerella forsythia, Treponema denticola, Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum.HSV, EBV and HCMV. Micro RNA 155were also assessed and compared between the groups. newlineResults: No significant difference was found in demographic variables (Age, BMI, SES) among the subjects. Whereas, elevated blood pressure was seen in groups 3 and 4 attributing to the preeclamptic condition in these two groups. We observed that all the PI, BOP, PPD, CAL, Tf, EBV and mir155 levels were highly significant in preeclamptic women with chronic periodontitis. were also seen to be elevated in the above patients. newlineThese results states that preeclamptic women with chronic periodontitis had more periodontal tissue destruction, microbial load in plaque and placental tissues (bacteria and virus) and mir155(placental tissues) owing to increased inflammation. newlineConclusion: Our results point to the fact that inflammation induced by periodontal pathogens pose high risk in preeclampsia development. Genetic studies in this field are advised to ascertain this phenomenon. newline