Stress Degradation Behaviour and Development Of Stability Indicating Rp Hplc Method For Simultaneous Estimation Of Combination Drug In Pharmaceutical Formulation

Abstract

This paper presents an extensive review on the methods and techniques employed in newlinepharmaceutical analysis, particularly focusing on the development and validation of newlineanalytical methods in the context of combination drug formulations. The introduction newlinediscusses the plethora of drugs available in various dosage forms and the instrumental newlinetechniques commonly used for their estimation, such as spectrophotometry, newlinechromatography, and high-performance liquid chromatography (HPLC). Method newlinedevelopment in analytical chemistry, encompassing both quantitative and qualitative newlineaspects, is highlighted as a challenging yet crucial process requiring thorough newlineliterature review and experimental finesse. The choice of analytical method is newlineinfluenced by factors like the nature of the material, desired accuracy, available newlinefacilities, and time constraints. newlineSubsequent sections delve into various analytical methods, including classical and newlineinstrumental techniques, with a focus on spectrophotometric methods and HPLC. newlineSpectrophotometric methods are praised for their simplicity, accuracy, and rapidity, newlinemaking them invaluable in pharmaceutical analysis. The paper also discusses the newlineprinciples and applications of HPLC, emphasizing its superiority in terms of speed, newlineresolution, reproducibility, and automation. The choice of HPLC column and mobile newlinephase is crucial and depends on the physical characteristics of the sample. newlineThe importance of method validation in ensuring the reliability and accuracy of newlineanalytical procedures is underscored, with a detailed discussion on parameters such as newlineprecision, accuracy, limit of detection, and specificity. Stability-indicating methods in newlineHPLC are highlighted as essential for differentiating between active pharmaceutical newlineingredients and degradation products during stability testing. Forced degradation newlinestudies under various conditions are recommended to evaluate the stability and newlinerobustness of analytical methods. newlineIn conclusion, the paper provides a comprehensive overview of analytical met