Studies on serratia marcescens lipase

Abstract

Psychrotrophs are cold adapted mesophilic organisms They newlinesucceed in maintaining adequate metabolic fluxes by the production of coldactive newlineenzymes which can efficiently catalyze biochemical reactions at low newlinetemperature with a higher specific activity Lipases EC 3 1 1 3 catalyze the newlinehydrolysis of triglycerides to free fatty acids and glycerol Biotechnologically newlinecold active lipases have gained considerable importance as biocatalyst as a newlineresult of their increasing use in the organic synthesis of chiral intermediates newlinePsychrotropic opportunistic pathogen Serratia marcescens secretes newlinea cold active lipase LipA that is extensively used in the production of chiral newlineprecursor for the coronary vasodilator diltiazem In the present study attempts newlinehave been made to understand the molecular adaptation of LipA to cold newlineconditions Further to prove the bi functional nature of LipA displayed by newlineamino acid sequences and finally to functionally characterize the amino and newlinecarboxy domains of LipA newlineLipA is the first enzyme of its family to have been studied for cold newlineAdaptation First objective of the work was to understand the kinetic newlineoptimization of LipA LipA was cloned expressed and purified newline