Prospecting for Selenium nanoparticle synthesizing microorganisms and optimization of a method for Selenium nanoparticle biosynthesis

Abstract

Nanotechnology is relatively young research field, which combines the principles of physical, chemical, biological and material sciences for fabrication of nano- sized materials with one or more dimensions. Nanotechnology not only offers quality products but also improves the manufacturing processes. Nanostructures (nanoparticles (NPs), nanowires (NWs), nanocomposites) are the structural and functional parts of nanotechnology. Nano-world is not new to the mankind. Selenium nanoparticles (Se NPs) are important nanomaterial s due to its enormous applications ranging from electronics to catalysis to biomedicines. Se NPs attracted considerable attention in biomedicine due to their high bioavailability and lower toxicity than that of other biologically utilizable form. Recent studies have indicated that Se NPs express antibacterial, antioxidant, antiprotozoal, and anticancer properties. Biomedical applications of Se NPs are increasing day by day so it became important to fabricate biocompatible Se NPs. A bacterium Pantoea agglomerans isolated from collected soil samples was explored for the synthesis of Se NPs in our laboratory. Pantoea agglomerans mediate synthesis of Se NPs under aerobic conditions both intra and extra cellularly. Synthesized NPs were characterized by UV- Vis spectroscopy, XRD, SEM and EDAX. After analysis it was found that Se NPs were amorphous, sizes is less than 100 nm. Optimization of different conditions including cultivation medium, temperature, pH, light intensity, concentration of Selenium dioxide (SeO2), and time of reaction for bio-synthesis of Se NPs via Pentoea agglomerence is carried out in current study. Enrofloxacin (EFX) is commonly used antibiotic in poultry, it has high bioavailability and effective against broad range of pathogenic microorganisms but it has been reported for immunological and oxidative stress. The protective effect of Se on EFX induced immunological suppression on cellular and humoral immunity and protective effect of Se NPs on EFX induced oxidative stress in broi