Green Synthesis Characterization and Evaluation of Magnesium and Zinc Oxide Nanoparticles on Bt Cotton Gossypium hirsutum L

Abstract

An investigation was carried out on green synthesis and characterization of newlinemagnesium and zinc oxide nanoparticles in Green Nanotechnology Labarotary, newlineUniversity of Agricultural Sciences, Dharwad, Karnataka. MgO and ZnO nanoparticles newlinewere successfully synthesized utilizing the reducing and capping potential of neem leaf newlineextract compared to amaranthus and hibiscus. Among the different methods of synthesis newlineviz., hot plate heating, heating with stirring and autoclave method, MgO and ZnO newlinenanoparticles were successfully synthesized through autoclave method (121and#263; and 15 newlinepsi for 30 minutes). The green synthesized MgO and ZnO nanoparticles were initially newlineidentified by the change in colour. Further, these nanoparticles were characterized by newlineUV-Visible spectroscopy that revealed an absorbance peak at 279 nm for MgO and 362 newlinenm for ZnO nanoparticles. Size of the synthesized particles are confirmed with particle newlinesize analyzer i.e., 48.1 nm for MgO and 60.9 nm for ZnO. Green synthesized MgO newlinenanoparticles were oval in shape and ZnO nanoparticles were rod shaped confirmed newlineusing scanning electron microscope (SEM). The purity and percent presence of newlineelemental magnesium (62.4 %) and zinc (82.6 %) nanoparticles were confirmed using newlineenergy dispersive X-ray (EDX) spectrum. After successful synthesis and newlinecharacterization of nanoparticles, pot experiment was conducted in polyhouse at newlineInstitute of Agri-Biotechnology (IABT), Dharwad, Karnataka, under controlled newlinecondition to standardize the optimum concentration of green synthesized magnesium newlineand zinc oxide nanoparticles for foliar application in Bt cotton. The data revealed that, newlinefoliar spray of green synthesized nano MgO at 500 ppm, ZnO at 500 ppm and MgO and newlineZnO each at 500 ppm was found to be optimum concentration for Bt cotton than other newlineconcentrations (100 and 250 ppm). The standardized optimum concentrations under newlineprotected condition were further studied under field condition including commercial newlinenano products and conventional magnesium and zinc sulphate. The field