Standardization and Preclinical study of Siddha Herbo Marine Formulation Sangu Parpam

Abstract

The raw drug sangu purchased from various part of Tamil Nadu. newline Raw drug and ingredients were authenticated by Marine Biology Regional Centre, Zoological survey of India, Chennai. newline Raw Sangu was purified as per the literature in three different methods and named as Spu I, Spu II and Spu III. newline By using purified samples Spu I, Spu II and Spu III, Sangu parpam (SP I, SP II, and SP III respectively) were prepared as per the proposal. newline Then the samples, Sangu before purification, sangu after purification and the three different preparation of Sangu parpam were analysed for standardization. newline The physicochemical analysis of the samples revealed the quality of purification process and preparation of the medicine. newline Sophisticated instrumental analysis reveals the quality and concentration of minerals and metals and powder properties of Raw Sangu and purified samples Spu I, II, III and prepared Sangu Parpam samples SP I, II , III. newline Acute toxicity study revealed Sangu Parpam I, II, III at the doses of 50mg/kg. newline 300mg/kg, 1000mg/kg and 2000mg/kg to the rats did not produce drug- related toxicity. No mortality was observed during the entire period of the study. Data obtained in this study indicated no significance physical and behavioral signs of any toxicity. newline Sub acute toxicity study revealed Sangu Parpam I, II, III can be considered safe, as it did not cause either any lethality or adverse changes with general behaviour of rats and also there were no observable detrimental effects (100 to 300mg/kg body weight) over a period of 28 days. newline Both acute and Sub acute toxicity studies of various preparation of Sangu parpam revealed safe in animals tested. newline Based upon physico chemical and sophisticated instrumental analysis SP II was validated for the Anti ulcer activity studies and the study revealed that SP II has significant anti ulcer activity in animal models tested. Thus this entire research work justifies and confirms the traditional claim Sangu parpam is one of the important medication for Peptic Ulcer.