evaluation of mitochondrial genome variations and single nucleotide polymorphisms of glutathione s transferase p1 gstp gene in the development of colorectal cancer

Abstract

In general population, the term cancer is so frightening and leads to severe distress and mental agony. In third world countries (like south Asian countries) the diagnosis and treatments that are available for cancer to the Common public are limited. Out of several cancers that affect human beings, colorectal cancer (CRC) is the second-highest prevalent cancer in females and third in males. CRC accounts for nearly 695,000 deaths, with a projection of over 14 lakhs (1.4 Million) of new cases each year till 2012. Colorectal cancer is characterized by abnormal growth of cells in appendix or rectum or colon. Pathogenesis and etiology of colorectal cancer remains elusive despite numerous Epidemiological studies categorized several factors for the cause of the disease. Due to the technological advancements in understanding the order of nucleotide bases (DNA sequencing) of nucleic Acids, there are several studies reported the nucleotide variation and colorectal cancer disease association. Here, in the present study we aimed to understand if there is any specific relation between organelle DNA i.e. Mitochondrial DNA D- Loop sequence variation and Mn-SOD expression in south Indian population by sequencing the colorectal cancer case and analogous control samples. Our high-resolution study indicated a total of 87 nucleotide sequence variations in studied colorectal cancer case and control samples. Out of 87 polymorphisms that were found, many are categorized as single base variations (Transitions Y or R). newline