Microbial production of protease enzyme in submerged solid state fermentation

Abstract

Present experimental study had emphasized on the biotransformation of industrial liquid wastes such as cheese whey and solid wastes like wheat bran, potato peel, carrot peel and mango peel to an industrial useful product, acid protease, and made this as an eco-friendly biosynthesis of enzyme production. Detailed methodologies were presented on the optimum production of acid protease by adopting various techniques viz. One Variable at a Time and statistical methodologies like factor screening method, Plackett-Burman Design and response surface methodologies, Box-Behnken and Central Composite Circumscribed designs by using inexpensive fermentation media components in submerged and solid state fermentation in order to fulfill the local demand of acid protease. This was the only study which dealt with the usage of same carbon sources (wheat rawa, wheat bran and wheat flour) for microbial production of extracellular protease in conventional approach and statistical methodology under solid state fermentation. The drawback of One Variable at Time was highlighted through the interaction of wheat bran with soybean meal and potato peel. newlineProteolytic fungal culture from soil disposed with abattoir waste were isolated and then used for acid protease production in both submerged and soild state fermentation from potent isolated fungal culture, Aspergillus spp. Disposal of abattoir waste onto the soil altered the physico-chemical, biological and enzymatic properties of the soil. Results of physical analysis showed that soil disposed with abattoir waste had grey color with bad smell and had high electrical conductivity and water holding capacity. The pH of disposed soil was less than control soil due to acidic nature of waste. Improved chemical properties of polluted soil were observed because of the presence of higher amounts of organic carbon content, potassium, and phosphate contents than control soil. High count of bacterial and fungal populations was found in test soil. With increased soil incubation period, protease and am