Evaluation of graphene oxide chitosan Composite as a carrier of sirna for Regulating BCL 2 expression on saos 2 Mg 63 cancer cells in vitro and In vivo studies

Abstract

In spite of the immune system s ability to identify the differences in newlinevarious protein structures at an atomic level, these tumour cells escape the newlinehost immune response and thereby, from destruction. Nevertheless, strategies newlineparticularly targeting cancer cells are a major challenge that most scientists try newlineto overcome despite having a humongous number of research groups working newlineon the various possible routes. It is worth mentioning that not many works are newlinefocused on targeting the over-expression of Bcl-2, one of the important newlineoncogenes, which has been witnessed in 50-70% of cancers that have been newlinereported. A biomaterial is defined traditionally as any material that is used for newlineeither medical or dental applications that contact the host cells in any form, newlinesuch as either a drug carrier device, or as a prosthesis towards the replacement newlineof damaged tissues. The main issues to be fulfilled by the biomaterial for newlineclinical applications are biocompatibility, bioactivity, ability to carry drug to newlinethe site, inflammatory responses, and other factors based on its application. newlineShort Interfering RNA (siRNA) is a topic of interest for scientists since it acts newlineas an efficient tool to target and silence specific mRNA proteins that are newlineresponsible for causing cancer, and thereby help in the destruction of tumour newlinecells. Since siRNA is negatively charged, it is an enormous challenge to newlineenable the siRNA to enter the cell cytoplasm and reach the target cells. newlineChitosan has attracted the attention of both industrial and academic newlineresearchers since the early 90s for various applications in the fields of drug newlinedelivery and tissue engineering. One of the most important parameters that newlinedifferentiates chitosan from other biodegradable polymers is its cationic newlineproperty, making it unique when compared with others. newline