Molecular mapping genetics and introgression of yellow rust resistance in bread wheat Triticum aestivum L

Abstract

The present research work entitled Molecular mapping, genetics and introgression of yellow rust resistance in bread wheat newline(Triticum aestivum L.) was undertaken with the objectives to study genetics of yellow rust resistance in resistant mutants, molecular newlinemapping of yellow rust resistance in F2 progenies and introgression of yellow rust resistance in agronomically superior lines. The newlineexperiment was carried out to screen sixty-six elite wheat germplasm lines for yellow rust resistance and various agro-morphological newlinetraits. Experimental lines were evaluated in six environments including RWRC, Malan under field conditions for three consecutive years newlineand at major hotspot locations, HAREC, Kukumseri and ICAR-IIWBR, Dalang Maidan. These lines were also evaluated for Seedling newlineResistance Test (SRT) using four prevalent pathotypes of yellow rust. Out of these experimental lines, yellow rust susceptible lines viz., newlineHD 2967, HS 240 and HS 295 were crossed with yellow rust resistant lines viz., HPW 368, HS 562 and TYRM2 (resistant mutant of HD newline2967) to create (S×R) cross combinations viz., HD 2967 × TYRM2, HD 2967 × HS 562, HS 240 × TYRM2 and HS 295 × HPW 368. newlineThese four crosses along with their segregating generations (P1, P2, F1, F2, B1, B2) were evaluated to study the genetics of yellow rust newlineresistance and generation mean analysis for various agro-morphological traits. newlineSegregation pattern of yellow rust resistance in the segregating generations of HD 2967 × TYRM2, HD 2967 × HS 562 and HS newline240 × TYRM2 revealed the monogenic and dominant nature of yellow rust resistance in TYRM 2 and HS 562 and therefore, these newlinegenotypes may be successfully utilized in the breeding programme to develop yellow rust resistant lines. Mean data of six different newlinegenerations i.e. P1, P2, F1, F2, B1, B2 from the four inter-varietal cross combinations were utilized for generation mean analysis using newlinecompact family block design. The analysis of variance exhibited significant mean squares due to families or crosses along with significan