Study of Anticancer Effect of Jasminum Sambac L Essential Oil Against Human Breast Cancer by Insilico Screening and Experimental Validation Through Invitro and Invivo Analysis

Abstract

This research delves on the exploration of Jasminum sambac essential oil newline(JEO) as a potential combatant against human breast cancer. Through a multifaceted newlineapproach, the study investigates the antimicrobial, anti-inflammatory, antioxidant, and newlineanticancer properties of JEO. Utilizing GC MS analysis, the essential oil was newlinescrutinized to identify its therapeutic constituents, considering their resemblance to newlinedrug-like molecules. Furthermore, network pharmacology analysis was employed to newlineelucidate the molecular mechanisms underlying the anticancer potential of JEO. Gene newlineontology and pathway enrichment analyses, coupled with compound target-pathway newlinenetwork construction using Cytoscape software, facilitated the identification of key newlinetargets and pathways involved in the therapeutic action of JEO. A molecular docking newlineanalysis was then conducted to investigate the interaction between selected newlinecompounds and breast cancer targets. Followed by network construction, the newlineanticancer potential of JEO was further validated through experiments in in vitro and newlinein vivo conditions. In human breast cancer cell lines (MCF-7 and MDA-MB-231 cells), newlineJEO exhibits notable cytotoxic effects that were observed and based on different assay newlineperformed, it indicates JEO can be an effective anticancer agent on in vitro newlinetreatments. Additionally, in vivo experiments using Sprague Dawley rat models newlineconfirmed the effectiveness of JEO in mitigating tumor growth induced by DMBA, newlinesupported by histopathological analysis. The promising results obtained underscore newlinethe potential therapeutic implications of JEO in striving against inducing cancer newlineapoptosis in the battle of this devastating disease newline