Isolation purification and characterization of a amylase and a glucosidase inhibitors

Abstract

Decreasing postprandial hyperglycemia is one of the practical approaches newlineconsidered for control of Type II Diabetes. This can be done with the help of and#945;-amylase newlineand and#945;-glucosidase inhibitors which inhibits enzymatic hydrolysis of carbohydrates after newlinefood intake and hence delay the absorption of glucose. newlineHowever, synthetic inhibitors available in market have serious side-effects. So, newlinemost of the population prefers natural inhibitors of these digestive enzymes which are newlineobtained from food source. Therefore, this study was focused towards the isolation, newlinepurification and characterization of and#945;-amylase and and#945;-glucosidase inhibitors from different newlineplant source for the management of hyperglycemia which is linked to Type II Diabetes as newlinewell as obesity. newlineIn present study, 20 different plant samples were extracted in six different newlinesolvents based on their polarity. Hence altogether we obtained 120 extracts which were newlinelater on screened for and#945;-amylase inhibitory potential by means of starch-agar diffusion newlineassay. The extracts with and#945;-amylase inhibitory potential were afterwards examined for thin newlinelayer chromatography (TLC) profiling which revealed different band patterns of newlinephytoconstituents. Subsequently, TLC-Autography was done which revealed specific newlinefractions of band patterns with potent and#945;-amylase inhibitors when treated with Gram s newlineiodine. The fractions after recollecting were checked for and#945;-glucosidase inhibition showed newlineconsiderable inhibition potential. newlineThe extracts screened after TLC-Autography, were then tested out for presence of newlinephytochemicals like proteins, carbohydrates, phenols and tannins, flavonoids, saponins, newlineglycosides, steroids, terpenoids, alkaloids, phlobatannins, fixed oils and fatty acids. newlineWhile, quantitative analysis of total phenolics was also carried out which revealed newlinedifferent concentrations when calculated in terms of gallic acid equivalent. newlineThe inhibitory percentage of both and#945;-amylase and and#945;-glucosidase was calculated newlinewhere altogether 66 extracts were found to be responsible for and#945;-amylas