Synthesis of nanoparticle from bio resources for management of blast diseases of rice

Abstract

Development of reliable and eco-friendly process for synthesis of newlinemetallic nanoparticle is an important steps in the field of nanotechnology. An effort was newlinemade for biosynthesis of gold nanoparticles from bioresources like microbes Aspergillus newlineniger, Metarhizium anisopliae, Lecanicillium lecannii, Purpeurocillium lilacinus and newlinebotanicals like Ocimum gratissimum, Acorus calamus, Aloe barbadensis and Azadirachta newlineindica. Chloroauric acid was added as precursor for the synthesis of gold nanoparticles. newlineStudy on in vitro efficacy of biosynthesized gold nanoparticle from bioresources was newlinetested at hundred percent concentration against Magnaporthe grisea and effective newlinesource was found to be Metarhizium anisopliae and Ocimum gratissimum. Further efficacy of newlinebiosynthesized gold nanoparticle from Metarhizium anisopliae and Ocimum gratissimum. was newlinetested against Magnaporthe grisea at three different concentrations (50, 100 and 150ppm) newlinecomparison was made with Tryclozole at 600 ppm. The result showed that the gold newlinenanoparticles at 150 ppm significantly inhibit the mycelia growth of the pathogens as newlinecompared to the Tryclozole at 600 ppm. The effective biosynthesized gold newlinenanoparticles was characterized by UV-Vis spectrophotometer, Dynamic Light newlineScattering (DLS), Fourier transform infrared spectrometer (FTIR), Zeta Sizer and newlineTransmission Electron Microscope (TEM). Formation of gold nanoparticles were newlineconfirmed by UV-VIS spectroscopy study with absorption peaks at 550 nm. FTIR study newlineshowed that synthesized gold nanoparticle has all the required functional groups like newlineOH, N-H, C-H and COO-. Study on surface properties of nanoparticles by using newlinezetasizer resulted that gold nanoparticle from Metarhizium anisopliae was found to be newlinenegative and were stable in nature with zeta potential value of -20.7 mV. DLS analysis newlineshowed that the average size of the biosynthesized gold nanoparticles is 32.54 nm with newlinepolydispersity index of 0.560. TEM study showed that shape of the biosynthesized newlinenanoparticle is from triangular to quasihedral and ...