Biochemical profile with special reference to acute phase protein and energy balance in crossbred cows during post partum uterine infection

Abstract

The present research work was conducted to evaluate the haemato biochemcial status on supplementation or without supplementation of bypass fat in peri parturient cows that developed or not developed uterine infection subsequently following calving. Study was also carried out to find the haemato-biochemical changes on different days of cycle in post partum cyclic cows with uterine infection along with treatment response. A total of 48 crossbred cows comprising 24 peri-partum and 24 post-partum cyclic cows at their third to fifth lactation were used as experimental animals in the newlinestudy. newlineHaemato-biochemical investigation carried out in four groups comprising six peri-partum cows in each, viz., non-supplemented with uterine infection (Group-I), non-supplemented without uterine infection (Group-II), supplemented with uterine infection (Group-III) and supplemented without uterine infection (Group-IV) on day -14, 2, 7 and 14 of peri-parturition revealed that the mean lymphocyte and neutrophil count varied significantly between groups and between days, while monocyte, eosinophil and basophil counts and haemoglobin levels differed significantly between days of observation. WBC and RBC counts and PCV level showed no significant variations. The newlinemean level of serum Hp, total protein, GGT, ALT, AST and ghrelin differed significantly between groups and between days while SAA varied significantly between days of sampling. No significant variations could be observed in serum albumin, IGF-I and Leptin levels. In non-supplemented and supplemented groups, 66.67 and 69.44 per cent uterine samples were positive for presence of bacteria. The frequency percentage of occurrence of bacterial isolates on day 2, 7 and 14 ostpartum was 75.00, 66.67 and 58.33 and, 66.67, 75.00 and 66.67 in non-supplemented and supplemented group respectively. Staphylococcus spp. and E. coli were isolated from both the groups, Staphylococcus spp. being predominant. newlineSerum ghrelin level had significant negative correlation with WBC count and serum total pr