Effect of arecoline and transforming growth Factor on myofibroblast transdifferentiation By assessment of mitochondrial bioenergetics A cell culture study

Abstract

Background: newlineOral sub mucous fibrosis (OSMF) is a condition wherein mesenchymal response to injury newlineare mediated through changes in fibroblast structure and function. Fibroblasts differentiate newlineto alpha smooth muscle actin positive (and#945;SMA+) myofibroblast, which is key feature newlineunderlying pathogenesis of OSMF. Studies highlight that myofibroblast phenotypes newlinevary from physiological to pathological conditions. Recently, role of transforming newlinegrowth factor and#946; (TGFand#946;) and mitochondrial reprogramming has been studied in organ fibrosis. newlineIn OSMF, the role of arecoline and TGFand#946; is well-recognized but there is a paucity newlinein literature regarding their effect in myofibroblast differentiation and mitochondrial newlinefunction. Thus, the present work aimed to evaluate the role of arecoline and TGFand#946; in newlinemyofibroblast differentiation by study of mitochondrial bioenergetics. newlineObjectives: newlinePrimary Objective: To evaluate role of arecoline and TGFand#946; on myofibroblast transdifferentiation newlineusing human oral fibroblast cell lines. newlineSecondary Objectives: newline1. To assess the effect of arecoline, TGFand#946; and combination of arecoline and TGFand#946; on newlinemyofibroblast transdifferentiation. newline2. To assess the mitochondrial bioenergetics in myofibroblast transdifferentiation newlineinduced by arecoline, TGFand#946; and combination of arecoline and TGF and#946;. newline3. To compare the mitochondrial bioenergetics in myofibroblast transdifferentiation newlineinduced by arecoline, TGFand#946; and combination of arecoline and TGFand#946; newline