Studies on Screening of Biologically Important Zn plus two and PPi Ions by a Chemosensor

Abstract

vii newlineABSTRACT newlineStudies on Screening of Biologically Important Zn²and#8314; and PPi Ions by a Chemosensor explores newlinethe development of advanced rhodamine-based chemosensors for the selective detection of newlinezinc (Zn²and#8314;) and pyrophosphate (PPi) ions, which are essential in numerous physiological newlineprocesses. The study responds to the need for highly sensitive, selective, and biologically newlinecompatible sensors capable of functioning effectively in complex biological environments. newlineThe research aimed to design and synthesize rhodamine-based chemosensors with optimized newlinebinding sites for Zn²and#8314; to ensure strong affinity, fluorescence response, and stability under newlinephysiological conditions. It also sought to develop chemosensors capable of selectively newlinedetecting PPi among structurally similar phosphate species, while further constructing dualfunction newlinesensors that can simultaneously and independently detect both Zn²and#8314; and PPi with newlineminimal cross-reactivity. These efforts were supported by rigorous biological validation, newlineincluding studies on cellular uptake, live cell imaging, biocompatibility, and testing in real newlinebiological samples. The methodology combined rational molecular design, spectroscopic and newlinecomputational analysis, and biological evaluation, forming a comprehensive approach for newlinesensor development. The results demonstrated excellent analytical performance, spectral newlineorthogonality, and reliable quantitative capabilities, establishing these sensors as valuable newlinetools for diagnostics and biological research. This work sets a strong foundation for future newlineinnovations in multi-analyte chemical sensing newline