Evaluation of probiotic derived biogenics and zingerone in pseudomonas aeruginosa induced murine surgical site infection model

Abstract

Pseudomonas aeruginosa is one of the frequently implicated pathogen in surgical site infection (SSI) due to its virulence phenotypes and biofilm forming ability. In the present study, cell free supernatant (CFS) and biogenics (Organic acids and/or precipitated protein fraction) of indigenous potential probiotics, Lactobacillus fermentum PUM and Pediococcus acidilactici BNS5B both alone and in combination with zingerone were found to inhibit biofilm, virulence factors and motility of P. aeruginosa PAO1. In vivo study revealed reduced P. aeruginosa burden, suppuration at surgical site vis-a-vis reduced levels of oxidants, pro-inflammatory cytokines, ameliorated anti-oxidants and healed infected surgical site compared with counter controls. In totality, combination of L. fermentum PUM derived biogenics and zingerone could be employed to treat P. aeruginosa induced SSI that needs to be correlated clinically. newline