Prevalence and Distribution of Staphylococcus from health care settings and Genotypic Identification of antibiotic resistant determinants

Abstract

Staphylococci are gram positive spherical bacteria which belong to family micrococcaceae. More than 50 species are registered in the database list of prokaryotic names with standing in nomenclature. Staphylococci are emerging nosocomial pathogen and causes superficial skin infection to deep seated infection in human begins. In the present study, Staphylococci were isolated from various clinical specimens and anterior nasal cavity of healthcare workers and patient visitors. Total 534 S.aureus and 586 CONS were isolated. Among CONS, S.epidermidis was isolated more followed by others. All the MRSA isolated were typed SCCmec (I to V), most of Staphylococcus carried SCCmec IV which is common in community acquired MRSA followed by other SCCmec types. Most of staphylococci showed resistant to Tetracycline followed by Cefoxitin. 1.11% isolates of S.aureus detected Linezolid antibiotic. Constitutive MLSb resistance was detected more in all the staphylococcus followed by inducible MLSB in D-test. Co-existence of more than two antibiotic resistant determinants and SCCmec types among the multidrug resistant Staphylococcus were common in our study. Emergence of antibiotic resistance is an emerging and growing problem globally. Our study estimates that the prevalence of antibiotic resistant in tertiary care hospital which would helps to avoid over usage of antibiotics in hospitals. newline newline newline