Nanohybrid DNA Sensor for the detection of Orientia tsutsugamushi causing scrub typhus

Abstract

Current research is designed to develop the DNA sensor-based point-of-care device for the newlinedetection of scrub typhus. Scrub typhus is a deadly disease transmitted by mites and caused by newlinethe bacteria Orientia tsutsugamushi (O. tsutsugamushi). It is a reappearing disease of the newlinetsutsugamushi triangle transmitted by the bite of chiggers (larval stage). Scrub typhus is difficult newlineto diagnose as its symptoms are similar to other fever-related illnesses. Enzyme-linked newlineimmunosorbent assay (ELISA), Immunofluorescence assay (IFA), Immunochromatographic test newline(ICT), Weil-Felix, Polymerase chain reaction (PCR), and Loop-mediated isothermal newlineamplification (LAMP) are all effective diagnostic procedures for scrub typhus. The Weil-Felix newlinetest was first employed in poor nations to screen for scrub typhus, but it was not recommended newlinedue to its lack of specificity and sensitivity. Other antibody-based methods such as IFA and newlineELISA are mostly used in the diagnosis of scrub typhus due to their high sensitivity and newlinespecificity, but they do not have the ability to detect infection in the early stages and require newlineconvalescent sampling for confirmation. On another deed, PCR-based methods combating the newlinelimitation of current diagnosis methods and are able to detect the infection at early stage with newlinehigh precision and sensitivity. The only limitation with this method is the lower recovery of newlinebacterial GDNA from patient samples and the genetic newline